Universität zu Köln

Milanez, Giovanni D., Masangkay, Frederick R., Scheid, Patrick, Dionisio, Joseph D., Somsak, Voravuth, Kotepui, Manas, Tangpong, Jitbanjong and Karanis, Panagiotis (2020). Acanthamoebaspecies isolated from Philippine freshwater systems: epidemiological and molecular aspects. Parasitol. Res., 119 (11). S. 3755 - 3762. NEW YORK: SPRINGER. ISSN 1432-1955

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Abstract

Free-living amoeba (FLA) research in the Philippines is still in its infancy but has, by far, demonstrated the presence of potentially pathogenic species.Acanthamoebamay cause sight-threatening and central nervous system infections to humans, yet its epidemiologic distribution from local environmental sources is yet to be defined. The present study aimed to provide a baseline epidemiologic distribution ofAcanthamoebaspp. in freshwater systems in the Philippines and establish potential pathogenicity of isolates through thermo-tolerance assay. A total of 63 water samples were collected from 13 freshwater systems all over the Philippine archipelago. The low-volume (50 ml) water samples were processed and cultured on non-nutrient agar lawned withEscherichia coliand observed for amoebic growth using light microscopy. Amoebic culture demonstrated 14.28% (9/63) positivity while further molecular testing of culture-positive plates usingAcanthamoeba-specific primers demonstrated 100% (9/9) confirmation ofAcanthamoebaspecies. Genotyping ofAcanthamoebaisolates revealed T1, T3, T4, T5, T7, T11, and T15 genotypes. Thermo-tolerance assay demonstrated that T5 and T7 genotypes were potentially pathogenic strains. The evidence of environmental distribution ofAcanthamoebaspp. in the freshwater systems in the Philippines and thermo-tolerance profile of isolates are significant aspects of amoeba study in public health and calls for initiatives in the dissemination of relevant information and the expansion of knowledge, awareness, and policies on pathogenic waterborne amoeba to mitigate, prevent, detect, and report cases of human infections.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Milanez, Giovanni D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Masangkay, Frederick R. UNSPECIFIED UNSPECIFIED UNSPECIFIED Scheid, Patrick UNSPECIFIED UNSPECIFIED UNSPECIFIED Dionisio, Joseph D. UNSPECIFIED UNSPECIFIED UNSPECIFIED Somsak, Voravuth UNSPECIFIED UNSPECIFIED UNSPECIFIED Kotepui, Manas UNSPECIFIED UNSPECIFIED UNSPECIFIED Tangpong, Jitbanjong UNSPECIFIED UNSPECIFIED UNSPECIFIED Karanis, Panagiotis UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-319257
DOI:	10.1007/s00436-020-06874-2
Journal or Publication Title:	Parasitol. Res.
Volume:	119
Number:	11
Page Range:	S. 3755 - 3762
Date:	2020
Publisher:	SPRINGER
Place of Publication:	NEW YORK
ISSN:	1432-1955
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language FREE-LIVING AMEBAS; CLIMATE-CHANGE; SPP.; IDENTIFICATION; INFECTION; LEGIONELLA; NAEGLERIA; STRAINS; HOSTS Multiple languages Parasitology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/31925