Milanez, Giovanni D., Masangkay, Frederick R., Scheid, Patrick, Dionisio, Joseph D., Somsak, Voravuth, Kotepui, Manas, Tangpong, Jitbanjong and Karanis, Panagiotis (2020). Acanthamoebaspecies isolated from Philippine freshwater systems: epidemiological and molecular aspects. Parasitol. Res., 119 (11). S. 3755 - 3762. NEW YORK: SPRINGER. ISSN 1432-1955
Full text not available from this repository.Abstract
Free-living amoeba (FLA) research in the Philippines is still in its infancy but has, by far, demonstrated the presence of potentially pathogenic species.Acanthamoebamay cause sight-threatening and central nervous system infections to humans, yet its epidemiologic distribution from local environmental sources is yet to be defined. The present study aimed to provide a baseline epidemiologic distribution ofAcanthamoebaspp. in freshwater systems in the Philippines and establish potential pathogenicity of isolates through thermo-tolerance assay. A total of 63 water samples were collected from 13 freshwater systems all over the Philippine archipelago. The low-volume (50 ml) water samples were processed and cultured on non-nutrient agar lawned withEscherichia coliand observed for amoebic growth using light microscopy. Amoebic culture demonstrated 14.28% (9/63) positivity while further molecular testing of culture-positive plates usingAcanthamoeba-specific primers demonstrated 100% (9/9) confirmation ofAcanthamoebaspecies. Genotyping ofAcanthamoebaisolates revealed T1, T3, T4, T5, T7, T11, and T15 genotypes. Thermo-tolerance assay demonstrated that T5 and T7 genotypes were potentially pathogenic strains. The evidence of environmental distribution ofAcanthamoebaspp. in the freshwater systems in the Philippines and thermo-tolerance profile of isolates are significant aspects of amoeba study in public health and calls for initiatives in the dissemination of relevant information and the expansion of knowledge, awareness, and policies on pathogenic waterborne amoeba to mitigate, prevent, detect, and report cases of human infections.
Item Type: | Journal Article | ||||||||||||||||||||||||||||||||||||
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URN: | urn:nbn:de:hbz:38-319257 | ||||||||||||||||||||||||||||||||||||
DOI: | 10.1007/s00436-020-06874-2 | ||||||||||||||||||||||||||||||||||||
Journal or Publication Title: | Parasitol. Res. | ||||||||||||||||||||||||||||||||||||
Volume: | 119 | ||||||||||||||||||||||||||||||||||||
Number: | 11 | ||||||||||||||||||||||||||||||||||||
Page Range: | S. 3755 - 3762 | ||||||||||||||||||||||||||||||||||||
Date: | 2020 | ||||||||||||||||||||||||||||||||||||
Publisher: | SPRINGER | ||||||||||||||||||||||||||||||||||||
Place of Publication: | NEW YORK | ||||||||||||||||||||||||||||||||||||
ISSN: | 1432-1955 | ||||||||||||||||||||||||||||||||||||
Language: | English | ||||||||||||||||||||||||||||||||||||
Faculty: | Unspecified | ||||||||||||||||||||||||||||||||||||
Divisions: | Unspecified | ||||||||||||||||||||||||||||||||||||
Subjects: | no entry | ||||||||||||||||||||||||||||||||||||
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URI: | http://kups.ub.uni-koeln.de/id/eprint/31925 |
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