Kotz, Sandra, Kullmann, Maximilian, Crone, Barbara, Kalayda, Ganna V., Jaehde, Ulrich and Metzger, Sabine (2015). Combination of two-dimensional gel electrophoresis and a fluorescent carboxyfluorescein-diacetate-labeled cisplatin analogue allows the identification of intracellular cisplatin-protein adducts. Electrophoresis, 36 (21). S. 2811 - 2820. HOBOKEN: WILEY. ISSN 1522-2683
Full text not available from this repository.Abstract
Cisplatin is one of the most widely used anticancer agents, but a major problem for successful chemotherapy is the development of drug resistance of tumor cells against cisplatin. Resistance to cisplatin is a multifactorial problem. A method to detect and identify intracellular cisplatin-protein adducts was developed using a fluorescent carboxyfluoresceindiacetate-labeled cisplatin analogue (CFDA-cisplatin), 2DE, and ESI-MS/MS. We identified several CFDA-cisplatin-protein adducts including members of the protein disulfide isomerase family (PDI). These are the first results of the detection of intracellular CFDA-cisplatin-protein adducts, which may help to understand the resistance mechanism of cisplatin.
Item Type: | Journal Article | ||||||||||||||||||||||||||||
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URN: | urn:nbn:de:hbz:38-387799 | ||||||||||||||||||||||||||||
DOI: | 10.1002/elps.201500188 | ||||||||||||||||||||||||||||
Journal or Publication Title: | Electrophoresis | ||||||||||||||||||||||||||||
Volume: | 36 | ||||||||||||||||||||||||||||
Number: | 21 | ||||||||||||||||||||||||||||
Page Range: | S. 2811 - 2820 | ||||||||||||||||||||||||||||
Date: | 2015 | ||||||||||||||||||||||||||||
Publisher: | WILEY | ||||||||||||||||||||||||||||
Place of Publication: | HOBOKEN | ||||||||||||||||||||||||||||
ISSN: | 1522-2683 | ||||||||||||||||||||||||||||
Language: | English | ||||||||||||||||||||||||||||
Faculty: | Unspecified | ||||||||||||||||||||||||||||
Divisions: | Unspecified | ||||||||||||||||||||||||||||
Subjects: | no entry | ||||||||||||||||||||||||||||
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URI: | http://kups.ub.uni-koeln.de/id/eprint/38779 |
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