Kalimuthu, Palraj, Fischer-Schrader, Katrin ORCID: 0000-0003-4532-8918, Schwarz, Gunter and Bernhardt, Paul V.
(2015).
A sensitive and stable amperometric nitrate biosensor employing Arabidopsis thaliana nitrate reductase.
J. Biol. Inorg. Chem., 20 (2).
S. 385 - 394.
NEW YORK:
SPRINGER.
ISSN 1432-1327
Abstract
Nitrate reductase (NR) from the plant Arabidopsis thaliana has been employed in the development of an amperometric nitrate biosensor that functions at physiological pH. The anion anthraquinone-2-sulfonate (AQ) is used as an effective artificial electron transfer partner for NR at a glassy carbon (GC) electrode. Nitrate is enzymatically reduced to nitrite and the oxidized form of NR is electrochemically reduced by the hydroquinone form of the mediator (AQH(2)). The GC/NR electrode shows a pronounced cathodic wave for nitrate reduction and the catalytic current increases linearly in the nitrate concentration range of 10-400 A mu M with a correlation coefficient of 0.989. Using an amperometric method, a low detection limit of 0.76 nM (S/N = 3) was achieved. The practical application of the present electrochemical biosensor was demonstrated by the determination of nitrate concentration in natural water samples and the results agreed well with a standard spectroscopic method.
Item Type: | Journal Article | ||||||||||||||||||||
Creators: |
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URN: | urn:nbn:de:hbz:38-406314 | ||||||||||||||||||||
DOI: | 10.1007/s00775-014-1171-0 | ||||||||||||||||||||
Journal or Publication Title: | J. Biol. Inorg. Chem. | ||||||||||||||||||||
Volume: | 20 | ||||||||||||||||||||
Number: | 2 | ||||||||||||||||||||
Page Range: | S. 385 - 394 | ||||||||||||||||||||
Date: | 2015 | ||||||||||||||||||||
Publisher: | SPRINGER | ||||||||||||||||||||
Place of Publication: | NEW YORK | ||||||||||||||||||||
ISSN: | 1432-1327 | ||||||||||||||||||||
Language: | English | ||||||||||||||||||||
Faculty: | Unspecified | ||||||||||||||||||||
Divisions: | Unspecified | ||||||||||||||||||||
Subjects: | no entry | ||||||||||||||||||||
Uncontrolled Keywords: |
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URI: | http://kups.ub.uni-koeln.de/id/eprint/40631 |
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