Universität zu Köln

Kalimuthu, Palraj, Fischer-Schrader, Katrin ORCID: 0000-0003-4532-8918, Schwarz, Gunter and Bernhardt, Paul V. (2015). A sensitive and stable amperometric nitrate biosensor employing Arabidopsis thaliana nitrate reductase. J. Biol. Inorg. Chem., 20 (2). S. 385 - 394. NEW YORK: SPRINGER. ISSN 1432-1327

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Abstract

Nitrate reductase (NR) from the plant Arabidopsis thaliana has been employed in the development of an amperometric nitrate biosensor that functions at physiological pH. The anion anthraquinone-2-sulfonate (AQ) is used as an effective artificial electron transfer partner for NR at a glassy carbon (GC) electrode. Nitrate is enzymatically reduced to nitrite and the oxidized form of NR is electrochemically reduced by the hydroquinone form of the mediator (AQH(2)). The GC/NR electrode shows a pronounced cathodic wave for nitrate reduction and the catalytic current increases linearly in the nitrate concentration range of 10-400 A mu M with a correlation coefficient of 0.989. Using an amperometric method, a low detection limit of 0.76 nM (S/N = 3) was achieved. The practical application of the present electrochemical biosensor was demonstrated by the determination of nitrate concentration in natural water samples and the results agreed well with a standard spectroscopic method.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kalimuthu, Palraj UNSPECIFIED UNSPECIFIED UNSPECIFIED Fischer-Schrader, Katrin UNSPECIFIED orcid.org/0000-0003-4532-8918 UNSPECIFIED Schwarz, Gunter UNSPECIFIED UNSPECIFIED UNSPECIFIED Bernhardt, Paul V. UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-406314
DOI:	10.1007/s00775-014-1171-0
Journal or Publication Title:	J. Biol. Inorg. Chem.
Volume:	20
Number:	2
Page Range:	S. 385 - 394
Date:	2015
Publisher:	SPRINGER
Place of Publication:	NEW YORK
ISSN:	1432-1327
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ELECTROCATALYTIC REDUCTION; BIOELECTROCATALYZED REDUCTION; DRINKING-WATER; ELECTRODE; NITRITE; METHEMOGLOBINEMIA; ELECTROCHEMISTRY; COMPOSITE; SEQUENCE; ACID Multiple languages Biochemistry & Molecular Biology; Chemistry, Inorganic & Nuclear Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/40631