Universität zu Köln

Zander, Esther, Fernandez-Gonzalez, Ana, Schleicher, Xenia, Dammhayn, Cathrin, Kamolvit, Witchuda, Seifert, Harald and Higgins, Paul G. ORCID: 0000-0001-8677-9454 (2014). Worldwide dissemination of acquired carbapenem-hydrolysing class D beta-lactamases in Acinetobacter spp. other than Acinetobacter baumannii. Int. J. Antimicrob. Agents, 43 (4). S. 375 - 378. AMSTERDAM: ELSEVIER SCIENCE BV. ISSN 1872-7913

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Abstract

The aim of this study was to identify acquired OXA-type carbapenemases in Acinetobacter spp. other than Acinetobacter baumannii. From a total of 453 carbapenem-susceptible and -resistant Acinetobacter isolates collected worldwide, 23 were positive for bla(OXA) genes by multiplex PCR. These isolates were identified as Acinetobacter pittn (n=18), Acinetobacter nosocomialis (n=2), Acinetobacter junn (n=1) and Acinetobacter genomic species 14TU/13BJ (n=2). The blaoxA genes and associated insertion sequence (IS) elements were sequenced by primer walking. In 11 of these isolates, sequencing of the PCR products revealed that they were false-positive for bla(OXA). The remaining 12 isolates, originating from Europe, Asia, South America, North America and South Africa, harboured OXA-23 (n=4), OXA-58 (n=5), OXA-40-like (n=1) and OXA143-like (n=1); oneA. plan isolate harboured both OXA-23 and OXA-58. IS elements were associated with blaoxA in 10 isolates. OXA multiplex PCR showed a high degree of false-positive results (47.8%), indicating that detection of blaoxA in non-baumannn Acinetobacter spp. should be confirmed using additional methods. (C) 2014 Elsevier BY, and the International Society of Chemotherapy. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Zander, Esther UNSPECIFIED UNSPECIFIED UNSPECIFIED Fernandez-Gonzalez, Ana UNSPECIFIED UNSPECIFIED UNSPECIFIED Schleicher, Xenia UNSPECIFIED UNSPECIFIED UNSPECIFIED Dammhayn, Cathrin UNSPECIFIED UNSPECIFIED UNSPECIFIED Kamolvit, Witchuda UNSPECIFIED UNSPECIFIED UNSPECIFIED Seifert, Harald UNSPECIFIED UNSPECIFIED UNSPECIFIED Higgins, Paul G. UNSPECIFIED orcid.org/0000-0001-8677-9454 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-442295
DOI:	10.1016/j.ijantimicag.2014.01.012
Journal or Publication Title:	Int. J. Antimicrob. Agents
Volume:	43
Number:	4
Page Range:	S. 375 - 378
Date:	2014
Publisher:	ELSEVIER SCIENCE BV
Place of Publication:	AMSTERDAM
ISSN:	1872-7913
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language GENETIC STRUCTURES; IDENTIFICATION; CALCOACETICUS; BLA(OXA-58); EXPRESSION; SPREAD Multiple languages Infectious Diseases; Microbiology; Pharmacology & Pharmacy Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/44229