Weber, Stefanie, Weiser, Barbara, Kemal, Kimdar S., Burger, Harold, Ramirez, Christina M., Korn, Klaus ORCID: 0000-0003-1891-2107, Anastos, Kathryn, Kaul, Rupert ORCID: 0000-0002-1586-4093, Kovacs, Colin and Doerfler, Walter (2014). Epigenetic analysis of HIV-1 proviral genomes from infected individuals: Predominance of unmethylated CpG's. Virology, 449. S. 181 - 190. SAN DIEGO: ACADEMIC PRESS INC ELSEVIER SCIENCE. ISSN 0042-6822

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Abstract

Efforts to cure HIV-1 infections aim at eliminating proviral DNA. Integrated DNA from various viruses often becomes methylated de novo and transcriptionally inactivated. We therefore investigated CpG methylation profiles of 55 of 94 CpG's (58.5%) in HIV-1 proviral genomes including ten CpG's in each LTR and additional CpG's in portions of gag, env, nef, rev, and tat genes. We analyzed 33 DNA samples from PBMC's of 23 subjects representing a broad spectrum of HIV-1 disease. In 22 of 23 HIV-1-infected individuals, there were only unmethylated CpG's regardless of infection status. In one long term nonprogressor, however, methylation of proviral DNA varied between 0 and 75% over an 11-year period although the CD4+ counts remained stable. Hence levels of proviral DNA methylation can fluctuate. The preponderance of unmethylated CpG's suggests that proviral methylation is not a major factor in regulating HIV-1 proviral activity in PBMC's. Unmethylated CpG's may play a role in HIV-1 immunopathogenesis. (C) 2013 Elsevier Inc. All rights reserved.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Weber, StefanieUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Weiser, BarbaraUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kemal, Kimdar S.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Burger, HaroldUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ramirez, Christina M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Korn, KlausUNSPECIFIEDorcid.org/0000-0003-1891-2107UNSPECIFIED
Anastos, KathrynUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kaul, RupertUNSPECIFIEDorcid.org/0000-0002-1586-4093UNSPECIFIED
Kovacs, ColinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Doerfler, WalterUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-448395
DOI: 10.1016/j.virol.2013.11.013
Journal or Publication Title: Virology
Volume: 449
Page Range: S. 181 - 190
Date: 2014
Publisher: ACADEMIC PRESS INC ELSEVIER SCIENCE
Place of Publication: SAN DIEGO
ISSN: 0042-6822
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
HUMAN-IMMUNODEFICIENCY-VIRUS; ACTIVE ANTIRETROVIRAL THERAPY; DE-NOVO METHYLATION; DNA METHYLATION; LATENT RESERVOIR; HAMSTER-CELLS; FOREIGN DNA; T-CELLS; B-CELLS; EXPRESSIONMultiple languages
VirologyMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/44839

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