Universität zu Köln

Wang, Lu, Cui, Yurong, Tang, Ming, Hu, Xinwu, Luo, Hongyan, Hescheler, Juergen and Xi, Jiaoya (2014). Puerarin Facilitates T-Tubule Development of Murine Embryonic Stem Cell-Derived Cardiomyocytes. Cell. Physiol. Biochem., 34 (2). S. 383 - 393. BASEL: KARGER. ISSN 1421-9778

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Abstract

Aims: The embryonic stem cell derived cardiomyocytes (ES-CM) is one of the promising cell sources for repopulation of damaged myocardium. However, ES-CMs present immature structure, which impairs their integration with host tissue and functional regeneration. This study used murine ES-CMs as an in vitro model of cardiomyogenesis to elucidate the effect of puerarin, the main compound found in the traditional Chinese medicine the herb Radix puerariae, on t-tubule development of murine ES-CMs. Methods: Electron microscope was employed to examine the ultrastructure. The investigation of transverse tubules (t-tubules) was performed by Di-8-ANEPPS staining. Quantitative real-time PCR was utilized to study the transcript level of genes related to t-tubule development. Results: We found that long-term application of puerarin throughout cardiac differentiation improved myofibril array and sarcomeres formation, and significantly facilitated t-tubules development of ES-CMs. The transcript levels of caveolin-3, amphiphysin-2 and junctophinlin-2, which are crucial for the formation and development of t-tubules, were significantly upregulated by puerarin treatment. Furthermore, puerarin repressed the expression of miR-22, which targets to caveolin-3. Conclusion: Our data showed that puerarin facilitates t-tubule development of murine ES-CMs. This might be related to the repression of miR-22 by puerarin and upregulation of Cav3, Bin1 and JP2 transcripts. Copyright (C) 2014 S. Karger AG, Basel

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Wang, Lu UNSPECIFIED UNSPECIFIED UNSPECIFIED Cui, Yurong UNSPECIFIED UNSPECIFIED UNSPECIFIED Tang, Ming UNSPECIFIED UNSPECIFIED UNSPECIFIED Hu, Xinwu UNSPECIFIED UNSPECIFIED UNSPECIFIED Luo, Hongyan UNSPECIFIED UNSPECIFIED UNSPECIFIED Hescheler, Juergen UNSPECIFIED UNSPECIFIED UNSPECIFIED Xi, Jiaoya UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-449428
DOI:	10.1159/000363007
Journal or Publication Title:	Cell. Physiol. Biochem.
Volume:	34
Number:	2
Page Range:	S. 383 - 393
Date:	2014
Publisher:	KARGER
Place of Publication:	BASEL
ISSN:	1421-9778
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language SARCOPLASMIC-RETICULUM; FUNCTIONAL-PROPERTIES; MATURATION; CONTRACTION; BIOGENESIS; CAVEOLAE; COMPLEX; ROLES; HEART; RAT Multiple languages Cell Biology; Physiology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/44942