Zigrino, Paola ORCID: 0000-0002-7470-0064, Ayachi, Ouissam, Schild, Alexander, Kaltenberg, Jennifer, Zamek, Jan, Nischt, Roswitha, Koch, Manuel
ORCID: 0000-0002-2962-7814 and Mauch, Cornelia
(2012).
Loss of epidermal MMP-14 expression interferes with angiogenesis but not with re-epithelialization.
Eur. J. Cell Biol., 91 (10).
S. 748 - 757.
JENA:
ELSEVIER GMBH, URBAN & FISCHER VERLAG.
ISSN 0171-9335
Abstract
Synthesis and activation of matrix metalloproteinases during wound healing are important for remodeling the extracellular matrix and modulating various cellular functions. The membrane-type 1 matrix metalloproteinase (MMP-14) has been shown to play a key role during these processes. To analyze the function of epidermal-derived MMP-14 during skin repair we generated mice lacking MMP-14 expression in the epidermis (MMP-14(ep-/-)). These mice displayed overall normal skin morphology and epidermal differentiation patterns. Wound repair in MMP-14(ep-/-) followed the same kinetics as in wild type mice (MMP-14(ep+/+)), and infiltration of neutrophils, leukocytes, and macrophages into the wound site was comparable. Microscopic analysis showed no altered re-epithelialization in the absence of epidermal MMP-14. Furthermore, epidermal differentiation at the end of the repair process and scar formation was normal. However, at day 14 post wounding, sustained angiogenesis was observed in MMP-14(ep-/-) mice in contrast to control mice. Interestingly, decreased levels of endostatin were detected in wound lysates of MMP-14(ep-/-) mice as well as in cultured keratinocytes. Taken together, these data indicate that MMP-14 expression in keratinocytes is dispensable for skin homeostasis and repair, but plays a crucial role in the epidermal-dermal crosstalk leading to modulation of vessel density. (C) 2012 Elsevier GmbH. All rights reserved.
Item Type: | Journal Article | ||||||||||||||||||||||||||||||||||||
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URN: | urn:nbn:de:hbz:38-481261 | ||||||||||||||||||||||||||||||||||||
DOI: | 10.1016/j.ejcb.2012.05.003 | ||||||||||||||||||||||||||||||||||||
Journal or Publication Title: | Eur. J. Cell Biol. | ||||||||||||||||||||||||||||||||||||
Volume: | 91 | ||||||||||||||||||||||||||||||||||||
Number: | 10 | ||||||||||||||||||||||||||||||||||||
Page Range: | S. 748 - 757 | ||||||||||||||||||||||||||||||||||||
Date: | 2012 | ||||||||||||||||||||||||||||||||||||
Publisher: | ELSEVIER GMBH, URBAN & FISCHER VERLAG | ||||||||||||||||||||||||||||||||||||
Place of Publication: | JENA | ||||||||||||||||||||||||||||||||||||
ISSN: | 0171-9335 | ||||||||||||||||||||||||||||||||||||
Language: | English | ||||||||||||||||||||||||||||||||||||
Faculty: | Unspecified | ||||||||||||||||||||||||||||||||||||
Divisions: | Unspecified | ||||||||||||||||||||||||||||||||||||
Subjects: | no entry | ||||||||||||||||||||||||||||||||||||
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URI: | http://kups.ub.uni-koeln.de/id/eprint/48126 |
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