Universität zu Köln

Zigrino, Paola ORCID: 0000-0002-7470-0064, Ayachi, Ouissam, Schild, Alexander, Kaltenberg, Jennifer, Zamek, Jan, Nischt, Roswitha, Koch, Manuel ORCID: 0000-0002-2962-7814 and Mauch, Cornelia (2012). Loss of epidermal MMP-14 expression interferes with angiogenesis but not with re-epithelialization. Eur. J. Cell Biol., 91 (10). S. 748 - 757. JENA: ELSEVIER GMBH, URBAN & FISCHER VERLAG. ISSN 0171-9335

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Abstract

Synthesis and activation of matrix metalloproteinases during wound healing are important for remodeling the extracellular matrix and modulating various cellular functions. The membrane-type 1 matrix metalloproteinase (MMP-14) has been shown to play a key role during these processes. To analyze the function of epidermal-derived MMP-14 during skin repair we generated mice lacking MMP-14 expression in the epidermis (MMP-14(ep-/-)). These mice displayed overall normal skin morphology and epidermal differentiation patterns. Wound repair in MMP-14(ep-/-) followed the same kinetics as in wild type mice (MMP-14(ep+/+)), and infiltration of neutrophils, leukocytes, and macrophages into the wound site was comparable. Microscopic analysis showed no altered re-epithelialization in the absence of epidermal MMP-14. Furthermore, epidermal differentiation at the end of the repair process and scar formation was normal. However, at day 14 post wounding, sustained angiogenesis was observed in MMP-14(ep-/-) mice in contrast to control mice. Interestingly, decreased levels of endostatin were detected in wound lysates of MMP-14(ep-/-) mice as well as in cultured keratinocytes. Taken together, these data indicate that MMP-14 expression in keratinocytes is dispensable for skin homeostasis and repair, but plays a crucial role in the epidermal-dermal crosstalk leading to modulation of vessel density. (C) 2012 Elsevier GmbH. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Zigrino, Paola UNSPECIFIED orcid.org/0000-0002-7470-0064 UNSPECIFIED Ayachi, Ouissam UNSPECIFIED UNSPECIFIED UNSPECIFIED Schild, Alexander UNSPECIFIED UNSPECIFIED UNSPECIFIED Kaltenberg, Jennifer UNSPECIFIED UNSPECIFIED UNSPECIFIED Zamek, Jan UNSPECIFIED UNSPECIFIED UNSPECIFIED Nischt, Roswitha UNSPECIFIED UNSPECIFIED UNSPECIFIED Koch, Manuel UNSPECIFIED orcid.org/0000-0002-2962-7814 UNSPECIFIED Mauch, Cornelia UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-481261
DOI:	10.1016/j.ejcb.2012.05.003
Journal or Publication Title:	Eur. J. Cell Biol.
Volume:	91
Number:	10
Page Range:	S. 748 - 757
Date:	2012
Publisher:	ELSEVIER GMBH, URBAN & FISCHER VERLAG
Place of Publication:	JENA
ISSN:	0171-9335
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language TYPE-1 MATRIX-METALLOPROTEINASE; FUNCTIONAL-CHARACTERIZATION; INHIBITOR ENDOSTATIN; EXTRACELLULAR-MATRIX; ENDOTHELIAL-CELLS; TRANSGENIC MICE; COLLAGEN-XVIII; WOUND REPAIR; RAT SKIN; SURVIVAL Multiple languages Cell Biology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/48126