Universität zu Köln

Wolf, Sylvi, Rataj, Felicitas, Zierau, Oliver, Ostermann, Kai, Diel, Patrick, Parr, Maria Kristina ORCID: 0000-0001-7407-8300, Vollmer, Guenter and Roedel, Gerhard (2010). A novel combined approach to detect androgenic activities with yeast based assays in Schizosaccharomyces pombe and Saccharomyces cerevisiae. Toxicol. Lett., 199 (3). S. 410 - 416. CLARE: ELSEVIER IRELAND LTD. ISSN 1879-3169

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Abstract

We describe the construction and validation of novel test systems for detecting androgenic activities using a combination of the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe. By applying the reporter enhanced Green Fluorescent Protein (EGFP) the incubation time could be reduced to only 24 h if compared to the classical p-galactosidase reporter (48 h). Both yeast systems were validated by analyzing the effects of seven androgens as well as five anti-androgens. One androgen (stanozolol) could be detected ten times more sensitive in S. cerevisiae than in S. pombe. Three of the five anti-androgenic substances showed no or only a slight effect in both yeast assays. The other two anti-androgens could be detected much better in S. pombe. Additionally, we could show that both yeast assays tolerated 10% urine within the media and still were capable to detect dihydrotestosterone at a concentration of 10(-8) M suggesting the use of the assays for applied doping pre-screening. In summary, the novel androgen-sensitive yeast assays have a large potential for various applications, e.g. as pre-screening in doping analysis or cattle feeding. A combination of both assays, exploiting these two phylogenetic very different yeasts, allows detection of the activity of a wide range of androgenic substances. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Wolf, Sylvi UNSPECIFIED UNSPECIFIED UNSPECIFIED Rataj, Felicitas UNSPECIFIED UNSPECIFIED UNSPECIFIED Zierau, Oliver UNSPECIFIED UNSPECIFIED UNSPECIFIED Ostermann, Kai UNSPECIFIED UNSPECIFIED UNSPECIFIED Diel, Patrick UNSPECIFIED UNSPECIFIED UNSPECIFIED Parr, Maria Kristina UNSPECIFIED orcid.org/0000-0001-7407-8300 UNSPECIFIED Vollmer, Guenter UNSPECIFIED UNSPECIFIED UNSPECIFIED Roedel, Gerhard UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-490639
DOI:	10.1016/j.toxlet.2010.10.007
Journal or Publication Title:	Toxicol. Lett.
Volume:	199
Number:	3
Page Range:	S. 410 - 416
Date:	2010
Publisher:	ELSEVIER IRELAND LTD
Place of Publication:	CLARE
ISSN:	1879-3169
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language HUMAN CELL-LINE; FISSION YEAST; RECEPTOR; EXPRESSION; REPORTER; VECTORS Multiple languages Toxicology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/49063