Universität zu Köln

Kohler, Maxie, Thomas, Andreas ORCID: 0000-0003-1199-0743, Walpurgis, Katja, Terlouw, Koen, Schaenzer, Wilhelm and Thevis, Mario (2010). Detection of His-tagged Long-R-3-IGF-I in a black market product. Growth Horm. IGF Res., 20 (5). S. 386 - 391. EDINBURGH: CHURCHILL LIVINGSTONE. ISSN 1096-6374

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Abstract

Objective: Performance-enhancing substances are illicitly used in elite or amateur sports and may be obtained from the black market due to a cheaper and easier availability. Although various studies have shown that black market products frequently do not contain the declared substances, enormous amounts of illegally produced and/or imported drugs are confiscated from athletes or at customs with alarming results concerning the outcome of the analyses of the ingredients. This case report describes the identification of His-tagged Long-R-3-IGF-I, which is usually produced for biochemical studies, in an injection vial. Design: The ingredients were isolated by immunoaffinity purification and identified by nano-UPLC, high-resolution/high accuracy mass spectrometry of the intact and trypsinated substance and by an enzyme-linked immunosorbent assay. Results: (Tandem) mass spectra characterized the protein as Long-R-3-IGF-I with a His(6)-tag attached to the C-terminus by the linker amino acids Leu-Glu. Conclusion: His-tags are commonly added to proteins during synthesis to allow a convenient and complete purification of the final product and His-tags are subsequently removed by specific enzymes when being attached to the N-terminus. The effects of His-tagged Long-R-3-IGF-I in humans have not been elucidated or described and the product may rather be a by-product from biochemical studies than synthesized for injection purposes. (C) 2010 Growth Hormone Research Society. Published by Elsevier Ltd. All rights reserved.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kohler, Maxie UNSPECIFIED UNSPECIFIED UNSPECIFIED Thomas, Andreas UNSPECIFIED orcid.org/0000-0003-1199-0743 UNSPECIFIED Walpurgis, Katja UNSPECIFIED UNSPECIFIED UNSPECIFIED Terlouw, Koen UNSPECIFIED UNSPECIFIED UNSPECIFIED Schaenzer, Wilhelm UNSPECIFIED UNSPECIFIED UNSPECIFIED Thevis, Mario UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-495613
DOI:	10.1016/j.ghir.2010.07.001
Journal or Publication Title:	Growth Horm. IGF Res.
Volume:	20
Number:	5
Page Range:	S. 386 - 391
Date:	2010
Publisher:	CHURCHILL LIVINGSTONE
Place of Publication:	EDINBURGH
ISSN:	1096-6374
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language GROWTH-FACTOR-I; MASS-SPECTROMETRIC DETERMINATION; IGF-I; TISSUE DISTRIBUTION; PLASMA-CLEARANCE; AFFINITY TAGS; HORMONE; INSULIN; PURIFICATION; PERFORMANCE Multiple languages Cell Biology; Endocrinology & Metabolism Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/49561