Universität zu Köln

Oestreicher, Iris, Almeida, Jorge Reis, Campean, Valentina, Rauh, Manfred ORCID: 0000-0002-4852-7157, Plank, Christian, Amann, Kerstin and Doetsch, Joerg (2010). Changes in 11 beta-hydroxysteroid dehydrogenase type 2 expression in a low-protein rat model of intrauterine growth restriction. Nephrol. Dial. Transplant., 25 (10). S. 3195 - 3204. OXFORD: OXFORD UNIV PRESS. ISSN 1460-2385

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Abstract

Methods. IUGR rats after maternal low-protein diet (n = 17) were compared with controls (n = 18). At 70 and 120 days of age, in situ distribution of 11 beta-HSD2 gene and protein expression was investigated by RT-PCR of microdissected tubules and immunohistochemistry. For in situ localization studies, double staining for 11 beta-HSD2 and calbindin was used. Serum levels of corticosterone and dehydrocorticosterone were measured by tandem mass spectrometry. Results. In IUGR rats, intra-arterial blood pressure significantly increased at Day 120 of life. Serum corticosterone/dehydrocorticosterone ratios and 11 beta-HSD2 mRNA in total kidney were not altered in IUGR animals. However, 11 beta-HSD2 mRNA concentration was significantly lower in microdissected tubuli of IUGR animals (Day 120: 0.18 +/- 0.14 vs 1.00 +/- 0.32 rel. units in controls; P < 0.05). In IUGR animals, immunostaining scores for 11 beta-HSD2 were significantly lower than in controls (P < 0.05). Double staining with calbindin showed lower expression of 11 beta-HSD2 in distal segments of the distal tubule. Conclusions. Our data indicate lower gene and protein expression of the pre-receptor enzyme 11 beta-HSD2 in IUGR animals when looking at specific renal compartments, but not in total kidney extracts. Thus, lower 11 beta-HSD2 as a mechanism for hypertension later in life might be missed without methods for in situ detection.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Oestreicher, Iris UNSPECIFIED UNSPECIFIED UNSPECIFIED Almeida, Jorge Reis UNSPECIFIED UNSPECIFIED UNSPECIFIED Campean, Valentina UNSPECIFIED UNSPECIFIED UNSPECIFIED Rauh, Manfred UNSPECIFIED orcid.org/0000-0002-4852-7157 UNSPECIFIED Plank, Christian UNSPECIFIED UNSPECIFIED UNSPECIFIED Amann, Kerstin UNSPECIFIED UNSPECIFIED UNSPECIFIED Doetsch, Joerg UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-496068
DOI:	10.1093/ndt/gfq354
Journal or Publication Title:	Nephrol. Dial. Transplant.
Volume:	25
Number:	10
Page Range:	S. 3195 - 3204
Date:	2010
Publisher:	OXFORD UNIV PRESS
Place of Publication:	OXFORD
ISSN:	1460-2385
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language BLOOD-PRESSURE; NEPHRON NUMBER; GLUCOCORTICOID ACTION; GENE-EXPRESSION; HYPERTENSION; PREGNANCY; CORTISOL; RECEPTOR; DISEASE Multiple languages Transplantation; Urology & Nephrology Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/49606