Universität zu Köln

Kalka, Katharina ORCID: 0000-0002-1697-1843, Keldenich, Zoe, Carstens, Henning, Walter, Bjorn, Rauen, Ursula, Ruhparwar, Arjang, Weymann, Alexander, Kamler, Markus, Reiner, Gerald and Koch, Achim ORCID: 0000-0002-1616-4873 (2022). Custodiol-MP for ex vivo lung perfusion - A comparison in a porcine model of donation after circulatory determination of death. Int. J. Artif. Organs, 45 (2). S. 162 - 174. LONDON: SAGE PUBLICATIONS LTD. ISSN 1724-6040

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Abstract

Introduction: Ex vivo lung perfusion (EVLP) is an established technique to evaluate and eventually recondition lungs prior to transplantation. Custodiol-MP (C-MP) solution is a new solution, designed for clinical machine perfusion, that has been used for kidneys. The aim of this study was to compare the effects of EVLP with Custodiol-MP on lung functional outcomes to the gold standard of EVLP with Steen Solution (TM). Material and Methods: In a porcine EVLP model of DCDD (Donation after Circulatory Determination of Death), lungs were perfused with Steen Solution (TM) (SS, n = 7) or Custodiol-MP solution supplemented with 55 g/l albumin (C-MP, n = 8). Lungs were stored cold for 4 h in low potassium dextran solution and subsequently perfused ex vivo for 4 h. During EVLP pulmonary gas exchange, activities of lactate dehydrogenase (LDH) and alkaline phosphatase (AP) as well as levels of lactate in the perfusate were recorded hourly. Results: Oxygenation capacity differed significantly between groups (averaged over 4 h: SS 274 +/- 178 mmHg; C-MP 284 +/- 151 mmHg p = 0.025). Lactate dehydrogenase activities and lactate concentrations were significantly lower in Custodiol-MP perfused lungs. In a porcine model of DCDD with 4 h of EVLP the use of modified Custodiol-MP as perfusion solution was feasible. The use of C-MP showed at least comparable lung functional outcomes to the use of Steen Solution(TM). Furthermore C-MP perfusion resulted in significantly lower lactate dehydrogenase activity and lactate levels in the perfusate and higher oxygenation capacity.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Kalka, Katharina UNSPECIFIED orcid.org/0000-0002-1697-1843 UNSPECIFIED Keldenich, Zoe UNSPECIFIED UNSPECIFIED UNSPECIFIED Carstens, Henning UNSPECIFIED UNSPECIFIED UNSPECIFIED Walter, Bjorn UNSPECIFIED UNSPECIFIED UNSPECIFIED Rauen, Ursula UNSPECIFIED UNSPECIFIED UNSPECIFIED Ruhparwar, Arjang UNSPECIFIED UNSPECIFIED UNSPECIFIED Weymann, Alexander UNSPECIFIED UNSPECIFIED UNSPECIFIED Kamler, Markus UNSPECIFIED UNSPECIFIED UNSPECIFIED Reiner, Gerald UNSPECIFIED UNSPECIFIED UNSPECIFIED Koch, Achim UNSPECIFIED orcid.org/0000-0002-1616-4873 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-582392
DOI:	10.1177/0391398821990663
Journal or Publication Title:	Int. J. Artif. Organs
Volume:	45
Number:	2
Page Range:	S. 162 - 174
Date:	2022
Publisher:	SAGE PUBLICATIONS LTD
Place of Publication:	LONDON
ISSN:	1724-6040
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CURRENT PRESERVATION TECHNOLOGY; MACHINE PERFUSION; FUTURE-PROSPECTS; INDUCED INJURY; COLD-STORAGE; TRANSPLANTATION; MECHANISMS; CELLS Multiple languages Engineering, Biomedical; Transplantation Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/58239