Universität zu Köln

Habenstein, Jens ORCID: 0000-0003-3589-6436, Schmitt, Franziska, Liessem, Sander, Ly, Alice ORCID: 0000-0002-0827-6840, Trede, Dennis, Wegener, Christian ORCID: 0000-0003-4481-3567, Predel, Reinhard, Rossler, Wolfgang and Neupert, Susanne (2021). Transcriptomic, peptidomic, and mass spectrometry imaging analysis of the brain in the ant Cataglyphis nadus. J. Neurochem., 158 (2). S. 391 - 413. HOBOKEN: WILEY. ISSN 1471-4159

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Abstract

Behavioral flexibility is an important cornerstone for the ecological success of animals. Social Cataglyphis nodus ants with their age-related polyethism characterized by age-related behavioral phenotypes represent a prime example for behavioral flexibility. We propose neuropeptides as powerful candidates for the flexible modulation of age-related behavioral transitions in individual ants. As the neuropeptidome of C. nodus was unknown, we collected a comprehensive peptidomic data set obtained by transcriptome analysis of the ants' central nervous system combined with brain extract analysis by Q-Exactive Orbitrap mass spectrometry (MS) and direct tissue profiling of different regions of the brain by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS. In total, we identified 71 peptides with likely bioactive function, encoded on 49 neuropeptide-, neuropeptide-like, and protein hormone prepropeptide genes, including a novel neuropeptide-like gene (fliktin). We next characterized the spatial distribution of a subset of peptides encoded on 16 precursor proteins with high resolution by MALDI MS imaging (MALDI MSI) on 14 mu m brain sections. The accuracy of our MSI data were confirmed by matching the immunostaining patterns for tachykinins with MSI ion images from consecutive brain sections. Our data provide a solid framework for future research into spatially resolved qualitative and quantitative peptidomic changes associated with stage-specific behavioral transitions and the functional role of neuropeptides in Cataglyphis ants.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Habenstein, Jens UNSPECIFIED orcid.org/0000-0003-3589-6436 UNSPECIFIED Schmitt, Franziska UNSPECIFIED UNSPECIFIED UNSPECIFIED Liessem, Sander UNSPECIFIED UNSPECIFIED UNSPECIFIED Ly, Alice UNSPECIFIED orcid.org/0000-0002-0827-6840 UNSPECIFIED Trede, Dennis UNSPECIFIED UNSPECIFIED UNSPECIFIED Wegener, Christian UNSPECIFIED orcid.org/0000-0003-4481-3567 UNSPECIFIED Predel, Reinhard UNSPECIFIED UNSPECIFIED UNSPECIFIED Rossler, Wolfgang UNSPECIFIED UNSPECIFIED UNSPECIFIED Neupert, Susanne UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-588671
DOI:	10.1111/jnc.15346
Journal or Publication Title:	J. Neurochem.
Volume:	158
Number:	2
Page Range:	S. 391 - 413
Date:	2021
Publisher:	WILEY
Place of Publication:	HOBOKEN
ISSN:	1471-4159
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language Biochemistry & Molecular Biology; Neurosciences Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/58867