Habenstein, Jens ORCID: 0000-0003-3589-6436, Schmitt, Franziska, Liessem, Sander, Ly, Alice ORCID: 0000-0002-0827-6840, Trede, Dennis, Wegener, Christian ORCID: 0000-0003-4481-3567, Predel, Reinhard, Rossler, Wolfgang and Neupert, Susanne (2021). Transcriptomic, peptidomic, and mass spectrometry imaging analysis of the brain in the ant Cataglyphis nadus. J. Neurochem., 158 (2). S. 391 - 413. HOBOKEN: WILEY. ISSN 1471-4159

Full text not available from this repository.

Abstract

Behavioral flexibility is an important cornerstone for the ecological success of animals. Social Cataglyphis nodus ants with their age-related polyethism characterized by age-related behavioral phenotypes represent a prime example for behavioral flexibility. We propose neuropeptides as powerful candidates for the flexible modulation of age-related behavioral transitions in individual ants. As the neuropeptidome of C. nodus was unknown, we collected a comprehensive peptidomic data set obtained by transcriptome analysis of the ants' central nervous system combined with brain extract analysis by Q-Exactive Orbitrap mass spectrometry (MS) and direct tissue profiling of different regions of the brain by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS. In total, we identified 71 peptides with likely bioactive function, encoded on 49 neuropeptide-, neuropeptide-like, and protein hormone prepropeptide genes, including a novel neuropeptide-like gene (fliktin). We next characterized the spatial distribution of a subset of peptides encoded on 16 precursor proteins with high resolution by MALDI MS imaging (MALDI MSI) on 14 mu m brain sections. The accuracy of our MSI data were confirmed by matching the immunostaining patterns for tachykinins with MSI ion images from consecutive brain sections. Our data provide a solid framework for future research into spatially resolved qualitative and quantitative peptidomic changes associated with stage-specific behavioral transitions and the functional role of neuropeptides in Cataglyphis ants.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Habenstein, JensUNSPECIFIEDorcid.org/0000-0003-3589-6436UNSPECIFIED
Schmitt, FranziskaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Liessem, SanderUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Ly, AliceUNSPECIFIEDorcid.org/0000-0002-0827-6840UNSPECIFIED
Trede, DennisUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Wegener, ChristianUNSPECIFIEDorcid.org/0000-0003-4481-3567UNSPECIFIED
Predel, ReinhardUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Rossler, WolfgangUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Neupert, SusanneUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
URN: urn:nbn:de:hbz:38-588671
DOI: 10.1111/jnc.15346
Journal or Publication Title: J. Neurochem.
Volume: 158
Number: 2
Page Range: S. 391 - 413
Date: 2021
Publisher: WILEY
Place of Publication: HOBOKEN
ISSN: 1471-4159
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
Biochemistry & Molecular Biology; NeurosciencesMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/58867

Downloads

Downloads per month over past year

Altmetric

Export

Actions (login required)

View Item View Item