Badbaran, Anita, Mailer, Reiner K., Dahlke, Christine, Woens, Jannis ORCID: 0000-0001-7435-9241, Fathi, Anahita
ORCID: 0000-0002-9351-9471, Mellinghoff, Sibylle C., Renne, Thomas
ORCID: 0000-0003-4594-5975, Addo, Marylyn M., Riecken, Kristoffer and Fehse, Boris
ORCID: 0000-0001-9780-7211
(2021).
Digital PCR to quantify ChAdOx1 nCoV-19 copies in blood and tissues.
Mol.Ther.-Methods Clin. Dev., 23.
S. 418 - 424.
CAMBRIDGE:
CELL PRESS.
ISSN 2329-0501
Abstract
Vaccination with the adenoviral-vector-based AstraZeneca ChAdOx1 nCov-19 (Vaxzevria) vaccine is efficient and safe. However, in rare cases vaccinated individuals developed life-threatening thrombotic complications, including thrombosis in cerebral sinus and splanchnic veins. Monitoring of the applied vector in vivo represents an important precondition to study the molecular mechanisms underlying vaccine-driven adverse effects now referred to as vaccine-induced immune thrombotic thrombocytopenia (VITT). We previously have shown that digital PCR (dPCR) is an excellent tool to quantify transgene copies in vivo. Here, we present a highly sensitive dPCR for in situ quantification of ChAdOx1 nCoV-19 copies. Using this method, we quantified vector copies in human plasma 24, 72, and 168 h post vaccination and in a variety of murine tissues in an experimental vaccination model 30 min post injection. We describe a method for high-sensitivity quantitative detection of ChAdOx1 nCoV-19 with possible implications to elucidate the mechanisms of severe ChAdOx1 nCov-19 vaccine complications.
Item Type: | Journal Article | ||||||||||||||||||||||||||||||||||||||||||||
Creators: |
|
||||||||||||||||||||||||||||||||||||||||||||
URN: | urn:nbn:de:hbz:38-594478 | ||||||||||||||||||||||||||||||||||||||||||||
DOI: | 10.1016/j.omtm.2021.10.002 | ||||||||||||||||||||||||||||||||||||||||||||
Journal or Publication Title: | Mol.Ther.-Methods Clin. Dev. | ||||||||||||||||||||||||||||||||||||||||||||
Volume: | 23 | ||||||||||||||||||||||||||||||||||||||||||||
Page Range: | S. 418 - 424 | ||||||||||||||||||||||||||||||||||||||||||||
Date: | 2021 | ||||||||||||||||||||||||||||||||||||||||||||
Publisher: | CELL PRESS | ||||||||||||||||||||||||||||||||||||||||||||
Place of Publication: | CAMBRIDGE | ||||||||||||||||||||||||||||||||||||||||||||
ISSN: | 2329-0501 | ||||||||||||||||||||||||||||||||||||||||||||
Language: | English | ||||||||||||||||||||||||||||||||||||||||||||
Faculty: | Unspecified | ||||||||||||||||||||||||||||||||||||||||||||
Divisions: | Unspecified | ||||||||||||||||||||||||||||||||||||||||||||
Subjects: | no entry | ||||||||||||||||||||||||||||||||||||||||||||
Uncontrolled Keywords: |
|
||||||||||||||||||||||||||||||||||||||||||||
URI: | http://kups.ub.uni-koeln.de/id/eprint/59447 |
Downloads
Downloads per month over past year
Altmetric
Export
Actions (login required)
![]() |
View Item |