Badbaran, Anita, Mailer, Reiner K., Dahlke, Christine, Woens, Jannis ORCID: 0000-0001-7435-9241, Fathi, Anahita ORCID: 0000-0002-9351-9471, Mellinghoff, Sibylle C., Renne, Thomas ORCID: 0000-0003-4594-5975, Addo, Marylyn M., Riecken, Kristoffer and Fehse, Boris ORCID: 0000-0001-9780-7211 (2021). Digital PCR to quantify ChAdOx1 nCoV-19 copies in blood and tissues. Mol.Ther.-Methods Clin. Dev., 23. S. 418 - 424. CAMBRIDGE: CELL PRESS. ISSN 2329-0501

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Abstract

Vaccination with the adenoviral-vector-based AstraZeneca ChAdOx1 nCov-19 (Vaxzevria) vaccine is efficient and safe. However, in rare cases vaccinated individuals developed life-threatening thrombotic complications, including thrombosis in cerebral sinus and splanchnic veins. Monitoring of the applied vector in vivo represents an important precondition to study the molecular mechanisms underlying vaccine-driven adverse effects now referred to as vaccine-induced immune thrombotic thrombocytopenia (VITT). We previously have shown that digital PCR (dPCR) is an excellent tool to quantify transgene copies in vivo. Here, we present a highly sensitive dPCR for in situ quantification of ChAdOx1 nCoV-19 copies. Using this method, we quantified vector copies in human plasma 24, 72, and 168 h post vaccination and in a variety of murine tissues in an experimental vaccination model 30 min post injection. We describe a method for high-sensitivity quantitative detection of ChAdOx1 nCoV-19 with possible implications to elucidate the mechanisms of severe ChAdOx1 nCov-19 vaccine complications.

Item Type: Journal Article
Creators:
CreatorsEmailORCIDORCID Put Code
Badbaran, AnitaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Mailer, Reiner K.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Dahlke, ChristineUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Woens, JannisUNSPECIFIEDorcid.org/0000-0001-7435-9241UNSPECIFIED
Fathi, AnahitaUNSPECIFIEDorcid.org/0000-0002-9351-9471UNSPECIFIED
Mellinghoff, Sibylle C.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Renne, ThomasUNSPECIFIEDorcid.org/0000-0003-4594-5975UNSPECIFIED
Addo, Marylyn M.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Riecken, KristofferUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Fehse, BorisUNSPECIFIEDorcid.org/0000-0001-9780-7211UNSPECIFIED
URN: urn:nbn:de:hbz:38-594478
DOI: 10.1016/j.omtm.2021.10.002
Journal or Publication Title: Mol.Ther.-Methods Clin. Dev.
Volume: 23
Page Range: S. 418 - 424
Date: 2021
Publisher: CELL PRESS
Place of Publication: CAMBRIDGE
ISSN: 2329-0501
Language: English
Faculty: Unspecified
Divisions: Unspecified
Subjects: no entry
Uncontrolled Keywords:
KeywordsLanguage
CELLSMultiple languages
Medicine, Research & ExperimentalMultiple languages
URI: http://kups.ub.uni-koeln.de/id/eprint/59447

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