Universität zu Köln

Golumba-Nagy, Viktoria, Kuehle, Johannes and Abken, Hinrich (2017). Genetic Modification of T Cells with Chimeric Antigen Receptors: A Laboratory Manual. Hum. Gene Ther. Methods, 28 (6). S. 302 - 310. NEW ROCHELLE: MARY ANN LIEBERT, INC. ISSN 1946-6544

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Abstract

Redirected T cells genetically modified with a chimeric antigen receptor (CAR) have induced spectacular remissions of refractory leukemia/lymphoma in early phase trials, attracting interest to use CAR T cells in a variety of other applications including solid cancer and nonmalignant diseases. However, extensive preclinical explorations demand highly effective and robust procedures for the genetic modification of blood T cells; the same applies for engineering with a recombinant T cell receptor. We present laboratory procedures in a step-by-step protocol to engineer human and mouse T cells with a CAR by -retro- or lentiviral transduction for further preclinical testing.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Golumba-Nagy, Viktoria UNSPECIFIED UNSPECIFIED UNSPECIFIED Kuehle, Johannes UNSPECIFIED UNSPECIFIED UNSPECIFIED Abken, Hinrich UNSPECIFIED UNSPECIFIED UNSPECIFIED
URN:	urn:nbn:de:hbz:38-209045
DOI:	10.1089/hgtb.2017.083
Journal or Publication Title:	Hum. Gene Ther. Methods
Volume:	28
Number:	6
Page Range:	S. 302 - 310
Date:	2017
Publisher:	MARY ANN LIEBERT, INC
Place of Publication:	NEW ROCHELLE
ISSN:	1946-6544
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language ADOPTIVE IMMUNOTHERAPY; LENTIVIRAL VECTOR; LEUKEMIA-VIRUS; LYMPHOCYTES; ACTIVATION; IMMUNOGLOBULIN; TRANSDUCTION; INTEGRATION; EXPRESSION; SYSTEM Multiple languages Biotechnology & Applied Microbiology; Genetics & Heredity; Medicine, Research & Experimental Multiple languages
Refereed:	Yes
URI:	http://kups.ub.uni-koeln.de/id/eprint/20904