Universität zu Köln

Badbaran, Anita, Mailer, Reiner K., Dahlke, Christine, Woens, Jannis ORCID: 0000-0001-7435-9241, Fathi, Anahita ORCID: 0000-0002-9351-9471, Mellinghoff, Sibylle C., Renne, Thomas ORCID: 0000-0003-4594-5975, Addo, Marylyn M., Riecken, Kristoffer and Fehse, Boris ORCID: 0000-0001-9780-7211 (2021). Digital PCR to quantify ChAdOx1 nCoV-19 copies in blood and tissues. Mol.Ther.-Methods Clin. Dev., 23. S. 418 - 424. CAMBRIDGE: CELL PRESS. ISSN 2329-0501

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Abstract

Vaccination with the adenoviral-vector-based AstraZeneca ChAdOx1 nCov-19 (Vaxzevria) vaccine is efficient and safe. However, in rare cases vaccinated individuals developed life-threatening thrombotic complications, including thrombosis in cerebral sinus and splanchnic veins. Monitoring of the applied vector in vivo represents an important precondition to study the molecular mechanisms underlying vaccine-driven adverse effects now referred to as vaccine-induced immune thrombotic thrombocytopenia (VITT). We previously have shown that digital PCR (dPCR) is an excellent tool to quantify transgene copies in vivo. Here, we present a highly sensitive dPCR for in situ quantification of ChAdOx1 nCoV-19 copies. Using this method, we quantified vector copies in human plasma 24, 72, and 168 h post vaccination and in a variety of murine tissues in an experimental vaccination model 30 min post injection. We describe a method for high-sensitivity quantitative detection of ChAdOx1 nCoV-19 with possible implications to elucidate the mechanisms of severe ChAdOx1 nCov-19 vaccine complications.

Item Type:	Journal Article
Creators:	Creators Email ORCID ORCID Put Code Badbaran, Anita UNSPECIFIED UNSPECIFIED UNSPECIFIED Mailer, Reiner K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Dahlke, Christine UNSPECIFIED UNSPECIFIED UNSPECIFIED Woens, Jannis UNSPECIFIED orcid.org/0000-0001-7435-9241 UNSPECIFIED Fathi, Anahita UNSPECIFIED orcid.org/0000-0002-9351-9471 UNSPECIFIED Mellinghoff, Sibylle C. UNSPECIFIED UNSPECIFIED UNSPECIFIED Renne, Thomas UNSPECIFIED orcid.org/0000-0003-4594-5975 UNSPECIFIED Addo, Marylyn M. UNSPECIFIED UNSPECIFIED UNSPECIFIED Riecken, Kristoffer UNSPECIFIED UNSPECIFIED UNSPECIFIED Fehse, Boris UNSPECIFIED orcid.org/0000-0001-9780-7211 UNSPECIFIED
URN:	urn:nbn:de:hbz:38-594478
DOI:	10.1016/j.omtm.2021.10.002
Journal or Publication Title:	Mol.Ther.-Methods Clin. Dev.
Volume:	23
Page Range:	S. 418 - 424
Date:	2021
Publisher:	CELL PRESS
Place of Publication:	CAMBRIDGE
ISSN:	2329-0501
Language:	English
Faculty:	Unspecified
Divisions:	Unspecified
Subjects:	no entry
Uncontrolled Keywords:	Keywords Language CELLS Multiple languages Medicine, Research & Experimental Multiple languages
URI:	http://kups.ub.uni-koeln.de/id/eprint/59447